Changes of serum TNF-α,IL-1β,IL-10,IL-13,lung ultrastructure and respiratory function in the early stage of pulmonary contusion / 实用医学杂志

Objective To investigate the changes of serum TNF-α,IL-1β,IL-10,IL-13,lung ultrastructure and respiratory function in the early stage of pulmonary contusion. Method 16 white rabbits were randomly assigned to lung contusion group and control group. Pulmonary contusion was established. Serum TNF-α,IL-1β, IL-10 and IL-13 level at 1 ,2 ,3 and 4 h after contusion were tested. Respiratory function data was obtained and ultrastructure was observed. Results In the early phase after pulmonary contusion ,the expression of IL-1β and TNF-αincreased while IL-10 and IL-13 decreased continuously. Lung ultrastructure changes included alveolar dam-age and massive erythrocyte sedimentation and inflammatory cell exudation in alveolar cavity. Alveolar ventilation and PO2 decreased significantly,P-AO2 increased continuously and lung compliance reduced. Conclusions In the early phase after pulmonary contusion ,the expression of IL-1β and TNF-α increases while IL-10 and IL-13 de-creases,lung ultrastructure is progressively destructed and lung function is severely damaged.

THE APPLICATION OF VIDEO-ASSISTDE THORACOSCOPE TECHNOLOGY IN THORAC-IC TRAUMA / 现代医院

Objective To discuss the value of video -assisted thoracoscope technology in diagnosis and treatment of thoracic trauma .Methods Form January 2009 to December 2013, a retrospective analysis was carried out on 43 patients with thoracic trauma .All the patients were treated with video -assisted thoracoscope technology . Results The procedure were successful and all the patients were cured out of hospital .Conclusion It is wide oper-ation indication, little harm, definite treapeutic effect, little complication and fast recovered to treat the patient with thoracic trauma using video -assisted thoracoscope technology , and it is a good choice for diagnosis and treatment in thoracic trauma.

Staged operations strategy for delayed spontaneous rupture of esophagus with empyema / 国际外科学杂志

ObjectiveTo summarize the effect of staged operations strategy for delayed spontaneous rupture of esophagus with empyema.MethodsThree patients in the department of thoracic surgery of the second affiliated hospital of Guangzhou medical university underwent staged operative treatment for delayed spontaneous rupture of esophagus with empyema.The primary operative procedure consisted of removal of empyema sac,T-tube drainage and jejunostomies.The second operative procedure included alimentary reconstruction with thoracic esophageal resection and exclusion,or with esophageal resection and exclusion later.ResultsAll cases were cured with mean hospitalization of 78.7 days.None of them had any dysphagia at followup of 5-14 months.ConclusionDelayed spontaneous rupture of esophagus with empyema can be managed safely and effectively through staged operations strategy.

Effect of nuclear factor-kappa B decoy oligodeoxynncleotides on respiratory function and cytokine expression after severe lung contusion in rabbits / 中华创伤杂志

Objective To explore the effect of nuclear factor-kappa B (NF-κB) decoy oligode-oxynucleotides (ODN) on respiratory function and expressions of IL-1β and IL-13 in serum following se-vere lung contusion in rabbits. Methods A total of 40 New-Zealand rabbits were randomly divided into four groups, ie, severe lung contusion group (Group A, n=12), severe lung contusion with NF-κB scrambled decoy ODN intervention group (Group B, n=12), severe lung contusion with sense NF- B de-coy ODN intervention group (Group C, n=12) and normal control group (Group D, n =4). After the contusion model was set up, the sense and scrambled NF-κB decoy ODN were infused into the rabbits via the jugular veins in different groups, with 20 g per experimental rabbit. After contusion, respiratory fre-quency, tidal volume, airway pressure, respiration flow rate curve and end expiration nitric oxide concen-tration were detected at 1, 2, 3 and 4 hours. The expressions of IL-1β and IL-13 in serum were observed by means of ELISA. Results After sense NF-κB decoy ODN intervention, alveolar ventilation, arteri-al PO_2 and pulmonary compliance were improved, compared with Group A and Group B, with statistical difference (P<0.01). The expression of IL-1β was decreased and that of IL-13 increased after sense NF-κB decoy ODN intervention to the severe lung contusion, compared with Groups A and B, with statis-tical difference (P <0.01). The expression of IL-1β was increased to peak level at 1 hour after contu-sion, which continued to the end of the experiment. While expression of IL-13 was decreased at 1 hour af-ter contusion and reached the minimum level at 4 hours. With intervention with sense decoy ODN, the in-creased expression of IL-1β was down-regulated, but expression of IL-13 remained at high level, with sta-tistical difference compared with Group A and Group B (P < 0.01). Conclusions Intervention with sense NF-κB decoy ODN can significantly protect the respiratory function, reduce the expression of IL-1β and increase expression of IL-13 after severe lung contusion.